Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Afadin/AF-6 Antibodies »

Product Summary for Afadin/AF-6 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1475 and 1525 of human AF6 (myeloid/lymphoid or mixed-lineage leukemia translocated to, 4 ) using the numbering given in entry BAA32484.1 (GeneID 4301).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Afadin/AF-6 Antibody

Western Blot: Afadin/AF-6 Antibody [NBP1-22996]

Western Blot: Afadin/AF-6 Antibody [NBP1-22996]

Western Blot: Afadin/AF-6 Antibody [NBP1-22996] - Detection of human and mouse AF6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AF6 antibody NBP1-22996 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: Afadin/AF-6 Antibody [NBP1-22996]

Western Blot: Afadin/AF-6 Antibody [NBP1-22996]

Western Blot: Afadin/AF-6 Antibody [NBP1-22996] - Whole cell lysate from HeLa cells. AF6 was also immunoprecipitated, albeit poorly, by rabbit anti-AF6 antibody NBP1-22997.
Afadin/AF-6 Antibody [NBP1-22996] - Detection of human AF6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AF6 antibody NBP1-22996 (lot NBP1-22996-2) used for IP at 6 ug per reaction. AF6 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-22996-1) and a another rabbit anti-AF6 antibody. For blotting immunoprecipitated AF6, NBP1-22996 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Afadin/AF-6 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Afadin/AF-6

AF6 was identified as part of a fusion with MLL (mixed lineage leukemia) in a t(6;11) genetic translocation. AF6 is also known as afadin (a splicing variant of AF6 found at adherins junction). AF6/afadin is an actin filament binding protein that binds to nectin at cadherin-based cell-cell adherens junctions and regulates cell-cell adhesion by mediating Ras signaling.

Alternate Names

AF-6, AF6, AFAD, MLLT4

Entrez Gene IDs

4301 (Human)

Gene Symbol

AFDN

UniProt

P55196

Additional Afadin/AF-6 Products

Product Documents for Afadin/AF-6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Afadin/AF-6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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