Afadin/AF-6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90218

Novus Biologicals, part of Bio-Techne
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NBP1-90218
NBP1-90218-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Afadin/AF-6 Antibodies »

Product Summary for Afadin/AF-6 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Afadin/AF-6 Antibody

Western Blot: Afadin/AF-6 Antibody [NBP1-90218]

Western Blot: Afadin/AF-6 Antibody [NBP1-90218]

Western Blot: Afadin/AF-6 Antibody [NBP1-90218] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Esophagus tissue
Immunocytochemistry/ Immunofluorescence: Afadin/AF-6 Antibody [NBP1-90218]

Immunocytochemistry/ Immunofluorescence: Afadin/AF-6 Antibody [NBP1-90218]

Immunocytochemistry/Immunofluorescence: Afadin/AF-6 Antibody [NBP1-90218] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles, plasma membrane & cell junctions.
Immunohistochemistry-Paraffin: Afadin/AF-6 Antibody [NBP1-90218]

Immunohistochemistry-Paraffin: Afadin/AF-6 Antibody [NBP1-90218]

Immunohistochemistry-Paraffin: Afadin/AF-6 Antibody [NBP1-90218] - Staining of human Small intestine shows moderate cytoplasmic positivity in glandular cells.
View 3 more images

Applications for Afadin/AF-6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Afadin/AF-6

AF6/Afadin was identified as part of a fusion with MLL (mixed lineage leukemia) in a t(6;11) genetic translocation. AF6/Afadin is also known as afadin (a splicing variant of AF6/Afadin found at adherins junction). AF6/afadin is an actin filament binding protein that binds to nectin at cadherin-based cell-cell adherens junctions and regulates cell-cell adhesion by mediating Ras signaling.

Alternate Names

AF-6, AF6, AFAD, MLLT4

Gene Symbol

AFDN

Additional Afadin/AF-6 Products

Product Documents for Afadin/AF-6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Afadin/AF-6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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