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AFAP Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81782CL1

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

AFAP antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AFAP. The immunogen is located within amino acids 30 - 80 of AFAP. Amino Acid Squence: FDVKDHAQKQETANSLPA

Specificity

Monomer and homomultimer of AFAP1 are known to exist; AFAP1 antibody is predicted to not cross-react with other AFAP family members.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for AFAP Antibody [CoraFluor™ 1]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: AFAP

AFAP, or Actin filament-associated protein 1, contains a 81 kDa and a 90 kDa isoform, and is involved in the manipulation of actin filaments to form network or bundle structures and the regulation of cell-matrix linkage. Disease research is currently being conducted with AFAP and its relation to familial adenomatous polyposis, adenocarcinoma, breast cancer, prostate adenocarcinoma, prostatitis, polyposis, prostate cancer, and carcinoma. This protein interacts with PRKCB, PRKCD, PRKCG, ACTC1, and PRKCZ during the processes of signal transduction regulation and mechanical stimulus response.

Long Name

Actin Filament-associated Protein, 110 kDa

Alternate Names

AFAP-110, AFAP1

Gene Symbol

AFAP1

Additional AFAP Products

Product Documents for AFAP Antibody [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AFAP Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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