AFF4 Antibody (2E12)

Novus Biologicals, part of Bio-Techne | Catalog # H00027125-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AFF4 Antibodies »

Product Specifications

Immunogen

AFF4 (NP_055238, 1 a.a. ~ 108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKW

Reactivity Notes

Human. Other species not tested.

Specificity

AFF4 - AF4/FMR2 family, member 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for AFF4 Antibody (2E12)

Western Blot: AFF4 Antibody (2E12) [H00027125-M01]

Western Blot: AFF4 Antibody (2E12) [H00027125-M01]

Western Blot: AFF4 Antibody (2E12) [H00027125-M01] - Analysis of AFF4 expression in transfected 293T cell line by AFF4 monoclonal antibody (M01), clone 2E12.Lane 1: AFF4 transfected lysate( 40.2 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: AFF4 Antibody (2E12) [H00027125-M01]

Immunohistochemistry-Paraffin: AFF4 Antibody (2E12) [H00027125-M01]

Immunohistochemistry-Paraffin: AFF4 Antibody (2E12) [H00027125-M01] - Analysis of monoclonal antibody to AFF4 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 1 ug/ml.
Sandwich ELISA: AFF4 Antibody (2E12) [H00027125-M01] - Detection limit for recombinant GST tagged AFF4 is approximately 0.03ng/ml as a capture antibody.

Applications for AFF4 Antibody (2E12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AFF4

AFF4, or AF4/FMR2 family member 4, contains a 127 kDa, 98 kDa, and 39 kDa isoform, and is involved in the process of improving the catalytic rate of RNA polymerase II transcription. Disease research is currently being conducted with AFF4 on its relation to acute lymphoblastic leukemia, as well as other types of leukemia. This protein interacts with SIAH1, CCNT1, MLLT3, MLLT1, and MED10 during the regulation of DNA-dependent transcription and the transcription of RNA polymerase II.

Alternate Names

AF4/FMR2 family, member 4, AF5Q31AF4/FMR2 family member 4, ALL1-fused gene from chromosome 5q31 protein, Major CDK9 elongation factor-associated protein, MCEFALL1 fused gene from 5q31, MGC75036, Protein AF-5q31

Entrez Gene IDs

27125 (Human)

Gene Symbol

AFF4

OMIM

604417 (Human)

UniProt

NP_055238 (Human)

Additional AFF4 Products

Product Documents for AFF4 Antibody (2E12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AFF4 Antibody (2E12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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