AFG3L2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35543
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 538-797 of human AFG3L2 (NP_006787.2).
Sequence:
DSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPGEKVAN
Sequence:
DSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPGEKVAN
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for AFG3L2 Antibody
Western Blot: AFG3L2 Antibody [NBP3-35543] -
Western Blot: AFG3L2 Antibody [NBP3-35543] - Western blot analysis of various lysates, using AFG3L2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry: AFG3L2 Antibody [NBP3-35543] -
Immunohistochemistry: AFG3L2 Antibody [NBP3-35543] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using AFG3L2 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunoprecipitation: AFG3L2 Antibody [NBP3-35543] -
Immunoprecipitation: AFG3L2 Antibody [NBP3-35543] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug AFG3L2 antibody. Western blot was performed from the immunoprecipitate using AFG3L2 antibody at a dilution of 1:1000.Applications for AFG3L2 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50 - 1:200
Immunoprecipitation
0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AFG3L2
Alternate Names
AFG3 (ATPase family gene 3, yeast)-like 2, AFG3 ATPase family gene 3-like 2 (S. cerevisiae), AFG3 ATPase family gene 3-like 2 (yeast), AFG3-like protein 2, ATPase family gene 3, yeast, EC 3.4.24, EC 3.4.24.-, FLJ25993, Paraplegin-like protein, SCA28, spinocerebellar ataxia 28
Gene Symbol
AFG3L2
Additional AFG3L2 Products
Product Documents for AFG3L2 Antibody
Product Specific Notices for AFG3L2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...