AG-2/AGR2 Antibody - Azide Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27392
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against synthetic peptides corresponding to amino acids 55-72 of human AGR2.
Specificity
Researchers are encouraged to use the sequence and accession number (NP_006399.1) that were used to design the peptide immunogen in order to assess potential cross-reactivity of the antibody to their species of interest. The AGR2 antibody is specific to AGR2 and does not recognize AGR3 (Verma et al, 2012)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AG-2/AGR2 Antibody - Azide Free
Western Blot: AG-2/AGR2 AntibodyAzide Free [NBP2-27392]
Western Blot: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - AGR2 expression in cancer cell lines of the biliary tract. AGR2 mRNA levels in six biliary tract cancer cell lines were measured by real-time RT-PCR. The AGR2 transcript levels were normalized against those of the GAPDH. Results shown are means +/- standard deviations of (1/2)CT of target - CT of GAPDH of three experiments. B. AGR2 protein expression was detected by western blot analysis with beta-actin as a loading control. The result shown is a representative of three experiments. Abbreviations: CBD, common bile duct; GB, gall bladder; AoV, ampulla of Vater; IHD, intrahepatic duct; HDB, hepatic duct bifurcation; WD, well differentiated; MD, moderately differentiated; PD, poorly differentiated [24]. Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-804), licensed under a CC-BY license. Image using the standard format of this product.Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody - Azide Free [NBP2-27392]
Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Formalin-fixed, paraffin-embedded human adenocarcinoma of the breast stained with AGR2 antibody (2 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.Western Blot: AG-2/AGR2 AntibodyAzide Free [NBP2-27392]
Western Blot: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Analysis of AGR2 using AGR2 antibody. MCF7 (A), Caco-2 (B) and human stomach lysate (C) probed with AGR2 antibody at 1:100. were used for this test. The higher molecular weight band of variable intensity at ~30 kDa is uncharacterized and may represent a form of AGR2.Applications for AG-2/AGR2 Antibody - Azide Free
Application
Recommended Usage
Immunohistochemistry-Frozen
1:10-1:500~
Immunohistochemistry-Paraffin
1:100
Immunoprecipitation
1:10-1:500
Simple Western
1:100
Western Blot
1:100
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.2 ml PBS
Format
Azide Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AG-2/AGR2
Long Name
Anterior Gradient 2 Homolog
Alternate Names
AG2, AGR2, GOB-4, HPC8, PDIA17
Gene Symbol
AGR2
Additional AG-2/AGR2 Products
Product Documents for AG-2/AGR2 Antibody - Azide Free
Product Specific Notices for AG-2/AGR2 Antibody - Azide Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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