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AG-2/AGR2 Antibody - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27392

Novus Biologicals, part of Bio-Techne
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NBP2-27392

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against synthetic peptides corresponding to amino acids 55-72 of human AGR2.

Specificity

Researchers are encouraged to use the sequence and accession number (NP_006399.1) that were used to design the peptide immunogen in order to assess potential cross-reactivity of the antibody to their species of interest. The AGR2 antibody is specific to AGR2 and does not recognize AGR3 (Verma et al, 2012)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AG-2/AGR2 Antibody - Azide Free

Western Blot: AG-2/AGR2 AntibodyAzide Free [NBP2-27392]

Western Blot: AG-2/AGR2 AntibodyAzide Free [NBP2-27392]

Western Blot: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - AGR2 expression in cancer cell lines of the biliary tract. AGR2 mRNA levels in six biliary tract cancer cell lines were measured by real-time RT-PCR. The AGR2 transcript levels were normalized against those of the GAPDH. Results shown are means +/- standard deviations of (1/2)CT of target - CT of GAPDH of three experiments. B. AGR2 protein expression was detected by western blot analysis with beta-actin as a loading control. The result shown is a representative of three experiments. Abbreviations: CBD, common bile duct; GB, gall bladder; AoV, ampulla of Vater; IHD, intrahepatic duct; HDB, hepatic duct bifurcation; WD, well differentiated; MD, moderately differentiated; PD, poorly differentiated [24]. Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-804), licensed under a CC-BY license. Image using the standard format of this product.
Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody - Azide Free [NBP2-27392]

Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody - Azide Free [NBP2-27392]

Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Formalin-fixed, paraffin-embedded human adenocarcinoma of the breast stained with AGR2 antibody (2 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Western Blot: AG-2/AGR2 AntibodyAzide Free [NBP2-27392]

Western Blot: AG-2/AGR2 AntibodyAzide Free [NBP2-27392]

Western Blot: AG-2/AGR2 Antibody - Azide Free [NBP2-27392] - Analysis of AGR2 using AGR2 antibody. MCF7 (A), Caco-2 (B) and human stomach lysate (C) probed with AGR2 antibody at 1:100. were used for this test. The higher molecular weight band of variable intensity at ~30 kDa is uncharacterized and may represent a form of AGR2.

Applications for AG-2/AGR2 Antibody - Azide Free

Application
Recommended Usage

Immunohistochemistry-Frozen

1:10-1:500~

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10-1:500

Simple Western

1:100

Western Blot

1:100
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml PBS

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AG-2/AGR2

hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR1 and AGR2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development(1). Although the roles of hAG-2 and hAG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21.

Long Name

Anterior Gradient 2 Homolog

Alternate Names

AG2, AGR2, GOB-4, HPC8, PDIA17

Gene Symbol

AGR2

Additional AG-2/AGR2 Products

Product Documents for AG-2/AGR2 Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AG-2/AGR2 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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