AG-3/AGR3 Antibody (AGR3.4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62196
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # AGR3.4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Purified human AGR3 protein
Specificity
The antibody DA-7 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for AG-3/AGR3 Antibody (AGR3.4)
Western Blot: Mouse Monoclonal AG-3/AGR3 Antibody (AGR3.4) [NBP2-62196] -
Western Blot: Mouse Monoclonal AG-3/AGR3 Antibody (AGR3.4) [NBP2-62196] - Western blotting analysis was performed on whole cell extracts (HOT lysis buffer) of MCF-7, HeLa, and Jurkat cell lines, mixed and heated (100C, 5 min) with reducing SDS-loading buffer. Samples were resolved using 15% SDS-PAGE gel.Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody AGR3.4 (1 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.AGR2/AGR3 molecules were detected at ~ 14-18 kDa in MCF-7 cell line.![Western Blot: AG-3/AGR3 Antibody (AGR3.4) [NBP2-62196]](https://resources.bio-techne.com/images/products/AG-3-AGR3-Antibody-AGR3-4-Western-Blot-NBP2-62196-img0001.jpg "Western Blot: AG-3/AGR3 Antibody (AGR3.4) [NBP2-62196]")
Western Blot: AG-3/AGR3 Antibody (AGR3.4) [NBP2-62196]
Western Blot: AG-3/AGR3 Antibody (AGR3.4) [NBP2-62196] - Analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.Applications for AG-3/AGR3 Antibody (AGR3.4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS and 0.2% (w/v) high grade BSA (protease free)
Preservative
0.9% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: AG-3
Long Name
Anterior Gradient 3 Homolog
Alternate Names
AG3, AGR3, BCMP11, PDIA18
Gene Symbol
AGR3
Additional AG-3 Products
Product Documents for AG-3/AGR3 Antibody (AGR3.4)
Product Specific Notices for AG-3/AGR3 Antibody (AGR3.4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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