AGAP1 Antibody (OTI4E8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72357

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4E8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all AGAP1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human AGAP1(NP_055729) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for AGAP1 Antibody (OTI4E8) - Azide and BSA Free

Immunohistochemistry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]

Immunohistochemistry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]

Immunohistochemistry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357] - Staining of paraffin-embedded Human tonsil using anti-AGAP1 mouse monoclonal antibody.
Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]

Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]

Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357] - Analysis of Jurkat cells, using anti-AGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]

Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]

Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGAP1.

Applications for AGAP1 Antibody (OTI4E8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AGAP1

CENTG2 belongs to an ADP-ribosylation factor GTPase-activating (ARF-GAP) protein family involved in membrane trafficand actin cytoskeleton dynamics (Nie et al., 2002 (PubMed 12388557)).(supplied by OMIM)

Alternate Names

AGAP-1, ANK repeat and PH domains 1, ArfGAP with GTPase domain, ankyrin repeat and PH domain 1, centaurin, gamma 2, Centaurin-gamma-2, CENTG2, cnt-g2, GTP-binding and GTPase-activating protein 1, KIAA1099arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1, MGC71657

Gene Symbol

AGAP1

Additional AGAP1 Products

Product Documents for AGAP1 Antibody (OTI4E8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AGAP1 Antibody (OTI4E8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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