Aggrecan Antibody (BC-3) [DyLight 550]
Novus Biologicals, part of Bio-Techne | Catalog # NB600-504R
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Bovine, Canine, Equine, Feline, Guinea Pig, Rabbit, Sheep
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
DyLight 550 (Excitation = 562 nm, Emission = 576 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BC-3
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
ARGSV... synthetic peptide conjugate.
Reactivity Notes
Cross-reacts with Human, Cow, Cat, Dog, Guinea pig, Horse, Pig, Rabbit, Rat and Sheep. Mouse reactivity reported in scientific literature (PMID: 27185069). Not yet tested in other species.
Localization
Secreted
Specificity
Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan.
Marker
Chondrocyte Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications for Aggrecan Antibody (BC-3) [DyLight 550]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections. This antibody can also be used with ELISA and Western blot, where it detects a variety of epitopes between 50 and 250 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Aggrecan
Alternate Names
ACAN, AGC1, CSPG1, CSPGCP, MSK16, SEDK
Gene Symbol
ACAN
Additional Aggrecan Products
Product Documents for Aggrecan Antibody (BC-3) [DyLight 550]
Product Specific Notices for Aggrecan Antibody (BC-3) [DyLight 550]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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