AGO1/2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18654
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Polyclonal antibody raised in rabbit against human AGO1/2 (eukaryotic translation initiation factor 2C1 and 2), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of both EIF2C1 and EIF2C2.
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for AGO1/2 Antibody
![Western Blot: AGO1/2 Antibody [NBP3-18654]](https://resources.bio-techne.com/images/products/AGO1-2-Antibody-Western-Blot-NBP3-18654-img0001.jpg "Western Blot: AGO1/2 Antibody [NBP3-18654]")
Western Blot: AGO1/2 Antibody [NBP3-18654]
Western Blot: AGO1/2 Antibody [NBP3-18654] - Figure 2. Western blot analysis using the antibody directed against AGO1/2 Whole cell extracts of U2OS cells (40 ug) were analysed by Western blot using the antibody against AGO1/2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 97 kDa); the marker (in kDa) is shown on the left.![ELISA: AGO1/2 Antibody [NBP3-18654]](https://resources.bio-techne.com/images/products/AGO1-2 Antibody-ELISA-NBP3-18654-img0002.jpg "ELISA: AGO1/2 Antibody [NBP3-18654]")
ELISA: AGO1/2 Antibody [NBP3-18654]
ELISA: AGO1/2 Antibody [NBP3-18654] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human AGO1/2 . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3,500.Applications for AGO1/2 Antibody
Application
Recommended Usage
ELISA
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Whole Antiserum
Formulation
Whole antiserum
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: AGO1/2
Alternate Names
argonaute 1, RISC catalytic component, argonaute 2, RISC catalytic component, argonaute RISC catalytic component 1, argonaute1, cancer susceptibility candidate 7 (non-protein coding), CASC7, EIF2C, eIF2C 1, eIF-2C 1, EIF2C1, EIF2C2, eukaryotic translation initiation factor 2C, 1, eukaryotic translation initiation factor 2C, 2, GERP95, Golgi Endoplasmic Reticulum protein 95 kDa, hAgo1, LESKRES, LINC00980, long intergenic non-protein coding RNA 980, PAZ Piwi domain protein, PPD, protein argonaute-1, protein argonaute-2, Protein slicer, Putative RNA-binding protein Q99, Q10, Q99
Gene Symbol
AGO1
UniProt
Additional AGO1/2 Products
Product Specific Notices for AGO1/2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...