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AGO1/EIF2C1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2817

Novus Biologicals, part of Bio-Techne
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NB100-2817

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-KNASYNLDPYIQEF corresponding to internal region according to NP_036331.1.

Specificity

This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for AGO1/EIF2C1 Antibody

Western Blot: AGO1/EIF2C1 Antibody [NB100-2817]

Western Blot: AGO1/EIF2C1 Antibody [NB100-2817]

Western Blot: AGO1/EIF2C1 Antibody [NB100-2817] - HEK293 overexpressing AGO1/EIF2C1 and probed with NB100-2817 (non-transfected HEK293 in lane B).
Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817]

Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817]

Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - Staining of paraffin embedded Human Placenta. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817]

Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817]

Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - Staining of paraffin embedded Human Small Intestine. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for AGO1/EIF2C1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/mL
Application Notes
Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32812257)WB: In transfected HEK293 transiently expressing human EIF2C1bands of approx. 110 Knockdown Validateda band and 35 Knockdown Validateda band are observed. The 110 Knockdown Validateda band is not observed in the non-transfected HEK293. The calculated molecular size is 97.2 Knockdown Validateda band according to NP_036331.1

Reviewed Applications

Read 1 review rated 4 using NB100-2817 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AGO1/EIF2C1

The AGO1/EIF2C1 gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-me

Alternate Names

AGO1Q99, argonaute 1, Argonaute1, DKFZp686M13167, EIF2C, eIF2C 1, eIF-2C 1, Eukaryotic translation initiation factor 2C 1, eukaryotic translation initiation factor 2C, 1, GERP95, Golgi Endoplasmic Reticulum protein 95 kDa, hAgo1, protein argonaute-1, Putative RNA-binding protein Q99

Entrez Gene IDs

26523 (Human)

Gene Symbol

AGO1

UniProt

Additional AGO1/EIF2C1 Products

Product Documents for AGO1/EIF2C1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AGO1/EIF2C1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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