Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # Agr 33

Concentration

1.0 mg/ml

View all Agrin Antibodies »

Product Specifications

Immunogen

Recombinant rat agrin, C-terminal construct.

Epitope

Epitope has been mapped near the 2nd laminin domain homology region and causes a marked reduction in the number of nicotinic acetylcholine receptor (nAChR) clusters. Insertion of an 8aa peptide at splicing site Z increases the clustering activity

Reactivity Notes

Cross-reacts with Mouse and Rat. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Synaptic basal lamina at the neuromuscular junction.

Specificity

detects all isoforms of agrin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Agrin Antibody (Agr 33)

Immunohistochemistry: Agrin Antibody (Agr 33) [NB120-12358]

Immunohistochemistry: Agrin Antibody (Agr 33) [NB120-12358]

Immunohistochemistry: Agrin Antibody (Agr 33) [NB120-12358] - Analysis of rat skeletal muscle probed with Agrin, mAb (Agr-33) at 1:100.

Applications for Agrin Antibody (Agr 33)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
ICC: Use at a concentration of 2 ug/ml. WB: Use at a concentration of 2 ug/ml. Predicted molecular weight: 208.6 kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Agrin

Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.

Alternate Names

AGRN

Entrez Gene IDs

11603 (Mouse); 25592 (Rat)

Gene Symbol

AGRN

Additional Agrin Products

Product Documents for Agrin Antibody (Agr 33)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Agrin Antibody (Agr 33)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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