Agrin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90209

Novus Biologicals, part of Bio-Techne
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NBP1-90209
NBP1-90209-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Agrin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Agrin Antibody

Immunocytochemistry/ Immunofluorescence: Agrin Antibody [NBP1-90209]

Immunocytochemistry/ Immunofluorescence: Agrin Antibody [NBP1-90209]

Immunocytochemistry/Immunofluorescence: Agrin Antibody [NBP1-90209] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209]

Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209]

Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209] - Staining of human Fallopian tube shows moderate positivity in basement membrane in glandular cells.
Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209]

Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209]

Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209] - Staining of human lung shows moderate membranous positivity in pneumocytes.
View 2 more images

Applications for Agrin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

Reported in scientific literature (PMID:32029550)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Agrin

Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.

Alternate Names

AGRN

Gene Symbol

AGRN

Additional Agrin Products

Product Documents for Agrin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Agrin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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