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AgRP/ART Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1540

Novus Biologicals, part of Bio-Techne
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NB100-1540

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence CRKLGTAMNPCSRT corresponding to C-Terminus according to NP_001129.1, NP_015531.1.

Epitope

C Terminus

Specificity

This antibody is expected to recognise both reported isoforms (as represented by NP_001129; NP_015531)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for AgRP/ART Antibody

Immunohistochemistry: AgRP/ART Antibody [NB100-1540]

Immunohistochemistry: AgRP/ART Antibody [NB100-1540]

Immunohistochemistry: AgRP/ART Antibody [NB100-1540] - Staining of PFA-perfused cryosection of Human Hypothalamus. Antibody at 0.05 ug/mL. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.

Applications for AgRP/ART Antibody

Application
Recommended Usage

Immunohistochemistry

0.05 - 0.1 ug/mL

Immunohistochemistry-Frozen

0.05 - 0.1 ug/mL

Peptide ELISA

Detection limit 1:32000
Application Notes
IHC-Fr: Human hypothalamus shows staining of a group of neuronal cell bodies in the human infundibular (arcuate) nucleus and their varicose axons.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AgRP/ART

AGRP is the endogenous antagonist of alpha-melanocyte stimulating hormone and has been shown to cause potent stimulation of food intake, and this protein is found in over 90% of Neuropeptide Y containing cells in rats.

Long Name

Agouti-related Protein

Alternate Names

ART

Entrez Gene IDs

181 (Human); 11604 (Mouse)

Gene Symbol

AGRP

Additional AgRP/ART Products

Product Documents for AgRP/ART Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AgRP/ART Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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