AGTR-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57073

Novus Biologicals, part of Bio-Techne
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NB100-57073
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Peptide ELISA, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all AGTR-1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EIQKNKPRNDDIFK corresponding to internal region according to NP_004826.2, NP_033611.1, NP_114038.1, NP_114438.1.

Specificity

All reported variants represent identical protein (NP_000676.1, NP_004826.2, NP_033611.1, NP_114038.1 and NP_114438.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AGTR-1 Antibody

Western Blot: AGTR-1 Antibody [NB100-57073]

Western Blot: AGTR-1 Antibody [NB100-57073]

Western Blot: AGTR-1 Antibody [NB100-57073] - Staining of Human Liver (A) + peptide (B), Mouse Liver (C) + peptide (D) lysate (35 ug protein in RIPA buffer). Antibody at 0.2 ug/mL. Detected by chemiluminescence.

Applications for AGTR-1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
WB: Approx 38 kDa band observed in Human and Mouse Liver lysates (calculated MW of 41.1 kDa according to Human NP_000676.1 and 40.9 kDa according to Mouse NP_796296.1) Additional bands of unknown identity were also observed at 45 and 75 kDa and were successfully blocked by incubation with the immunizing peptide. Use in Immunohistochemistry reported in scientific literature (PMID: 23335130).

Reviewed Applications

Read 1 review rated 1 using NB100-57073 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AGTR-1

Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. At least four transcript variants have been described for this gene. Additional variants have been described but their full-length nature has not been determined. The entire coding sequence is contained in the terminal exon and is present in all transcript variants.

Long Name

Angiotensin II Receptor Type 1

Alternate Names

AG2S, AGTR1, AT1, AT1AR, AT1BR, AT1R, AT2R1

Entrez Gene IDs

185 (Human); 11607 (Mouse); 24180 (Rat)

Gene Symbol

AGTR1

UniProt

P30556 (Human)

Additional AGTR-1 Products

Product Documents for AGTR-1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AGTR-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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