AHR Antibody (RPT1)
Novus Biologicals, part of Bio-Techne | Catalog # NB300-530
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Primate
Cited:
Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # RPT1
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of human AhR.
Specificity
Detects the aryl hydrocarbon receptor (AhR).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for AHR Antibody (RPT1)
Western Blot: AHR Antibody (RPT1) [NB300-530]
Western Blot: AHR Antibody (RPT1) [NB300-530] - Analysis of 20ug of HepG2, U2OS, and K562 whole cell lysates and 5uL of PageRuler Plus Prestained Protein Ladder per well.Immunocytochemistry/ Immunofluorescence: AHR Antibody (RPT1) [NB300-530]
Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.Immunohistochemistry-Paraffin: AHR Antibody (RPT1) [NB300-530]
Immunohistochemistry-Paraffin: AHR Antibody (RPT1) [NB300-530] - Cancer biopsies of deparaffinized Human breast carcinoma tissue at a dilution of 1:100.Applications for AHR Antibody (RPT1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:1000
Immunohistochemistry
1:10 - 1:100
Immunohistochemistry-Paraffin
1:10 - 1:100
Immunoprecipitation
1:10 - 1:500
Western Blot
1:500 - 1:2000
Application Notes
WB: Detects an approx. 95 kDa protein representing AhR from Hepa 1 cytosol. Although ab2770 can be used in IP procedures, clone RPT9 is recommended for this procedure.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AHR
Long Name
Aryl Hydrocarbon Receptor
Alternate Names
BHLHE76
Gene Symbol
AHR
UniProt
Additional AHR Products
Product Documents for AHR Antibody (RPT1)
Product Specific Notices for AHR Antibody (RPT1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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