AIF Antibody (22E9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59432

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 22E9

Format

BSA Free

Concentration

1 mg/ml

View all AIF Antibodies »

Product Specifications

Immunogen

Recombinant human AIF (98-609aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for AIF Antibody (22E9) - BSA Free

Western Blot: AIF Antibody (22E9)BSA Free [NBP2-59432]

Western Blot: AIF Antibody (22E9)BSA Free [NBP2-59432]

Western Blot: AIF Antibody (22E9) [NBP2-59432] - Lane 1: Mouse heart tissue lysates, Lane 2: Mouse liver tissue lysates
Immunocytochemistry/ Immunofluorescence: AIF Antibody (22E9)BSA Free [NBP2-59432]

Immunocytochemistry/ Immunofluorescence: AIF Antibody (22E9)BSA Free [NBP2-59432]

Immunocytochemistry/Immunofluorescence: AIF Antibody (22E9) [NBP2-59432] - Analysis of AIFM1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AIFM1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for AIF Antibody (22E9) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AIF

AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4).

Long Name

Apoptosis Inducing Factor

Alternate Names

AIFM1, COXPD6, PDCD8

Gene Symbol

AIFM1

Additional AIF Products

Product Documents for AIF Antibody (22E9) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AIF Antibody (22E9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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