AIF Antibody (5F0Y1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15759
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5F0Y1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 450-550 of human AIF (O95831). RRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AIF Antibody (5F0Y1)
Western Blot: AIF Antibody (5F0Y1) [NBP3-15759]
Western Blot: AIF Antibody (5F0Y1) [NBP3-15759] - Western blot analysis of extracts of various cell lines, using AIF antibody (NBP3-15759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Immunohistochemistry-Paraffin: AIF Antibody (5F0Y1) [NBP3-15759]
Immunohistochemistry-Paraffin: AIF Antibody (5F0Y1) [NBP3-15759] - Immunohistochemistry of paraffin-embedded human oophoroma using AIF antibody (NBP3-15759) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for AIF Antibody (5F0Y1)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AIF
Long Name
Apoptosis Inducing Factor
Alternate Names
AIFM1, COXPD6, PDCD8
Gene Symbol
AIFM1
Additional AIF Products
Product Documents for AIF Antibody (5F0Y1)
Product Specific Notices for AIF Antibody (5F0Y1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...