Aiolos/IKZF3 Antibody (OTI7E11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46048

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI7E11

Concentration

1 mg/ml

View all Aiolos/IKZF3 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-301 of human IKZF3 (NP_036613) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

57.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aiolos/IKZF3 Antibody (OTI7E11)

Western Blot: Aiolos/IKZF3 Antibody (OTI7E11) [NBP2-46048]

Western Blot: Aiolos/IKZF3 Antibody (OTI7E11) [NBP2-46048]

Western Blot: Aiolos/IKZF3 Antibody (7E11) [NBP2-46048] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IKZF3.
Immunohistochemistry: Aiolos/IKZF3 Antibody (OTI7E11) [NBP2-46048]

Immunohistochemistry: Aiolos/IKZF3 Antibody (OTI7E11) [NBP2-46048]

Immunohistochemistry: Aiolos/IKZF3 Antibody (7E11) [NBP2-46048] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Aiolos/IKZF3 Antibody (OTI7E11) [NBP2-46048]

Immunohistochemistry: Aiolos/IKZF3 Antibody (OTI7E11) [NBP2-46048]

Immunohistochemistry: Aiolos/IKZF3 Antibody (7E11) [NBP2-46048] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
View 1 more images

Applications for Aiolos/IKZF3 Antibody (OTI7E11)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aiolos/IKZF3

IKZF3 encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described. [provided by RefSeq]

Long Name

DNA-binding Protein Aiolos

Alternate Names

AIO, IKZF3, ZNFN1A3

Gene Symbol

IKZF3

Additional Aiolos/IKZF3 Products

Product Documents for Aiolos/IKZF3 Antibody (OTI7E11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Aiolos/IKZF3 Antibody (OTI7E11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.