AIP/ARA9 Antibody (PSH03-17)

Western Blot: AIP/ARA9 Antibody (PSH03-17) [NBP3-32012]

Western Blot: AIP/ARA9 Antibody (PSH03-17) [NBP3-32012] - Western blot analysis of AIP/ARA9 on different lysates with Rabbit anti-AIP/ARA9 antibody (NBP3-32012) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2:Jurkat cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: THP-1 cell lysate
Lane 5: HCT 1 cell lysate
Lane 6: MCF7 cell lysate
Lane 7: RAW264.7 cell lysate
Lane 8: NIH/3T3 cell lysate
Lane 9: C6 cell lysate
Lane 10: Mouse spleen tissue lysate
Lane 11: Rat spleen tissue lysate

Cell lysates/proteins at 20 ug/Lane.
Tissuel lysates/proteins at 40 ug/Lane.

Predicted band size: 38 kDa
Observed band size: 38 kDa

Exposure time: 1 minutes 2 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32012) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.