AKAP10 Antibody (8C10)

Novus Biologicals, part of Bio-Techne | Catalog # H00011216-M04

Novus Biologicals, part of Bio-Techne
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H00011216-M04
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 8C10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AKAP10 Antibodies »

Product Specifications

Immunogen

AKAP10 (NP_009133, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRGAGPSPRQSPRTLRPDPGPAMSFFRRKVKGKEQEKTSDVKSIKASISVHSPQKSTKNHALLEAAGPSHVAINAISANMDSFSSSRTATLKKQPSHMEA

Specificity

AKAP10 - A kinase (PRKA) anchor protein 10

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for AKAP10 Antibody (8C10)

Western Blot: AKAP10 Antibody (8C10) [H00011216-M04]

Western Blot: AKAP10 Antibody (8C10) [H00011216-M04]

Western Blot: AKAP10 Antibody (8C10) [H00011216-M04] - Analysis of AKAP10 expression in Raw 264.7.
Immunohistochemistry-Paraffin: AKAP10 Antibody (8C10) [H00011216-M04]

Immunohistochemistry-Paraffin: AKAP10 Antibody (8C10) [H00011216-M04]

Immunohistochemistry-Paraffin: AKAP10 Antibody (8C10) [H00011216-M04] - Analysis of monoclonal antibody to AKAP10 on formalin-fixed paraffin-embedded human testis. Antibody concentration 0.5 ug/ml
Western Blot: AKAP10 Antibody (8C10) [H00011216-M04]

Western Blot: AKAP10 Antibody (8C10) [H00011216-M04]

Western Blot: AKAP10 Antibody (8C10) [H00011216-M04] - Western blot analysis of AKAP10 over-expressed 293 cell line. Lane 2 cotransfected with AKAP10 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00011216-M04. GAPDH (36.1 kDa) used as loading control.
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Applications for AKAP10 Antibody (8C10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation, and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AKAP10

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction.

Alternate Names

A kinase (PRKA) anchor protein 10, AKAP-10, A-kinase anchor protein 10, mitochondrial, D-AKAP2, D-AKAP-2, Dual specificity A kinase-anchoring protein 2, dual-specificity A-kinase anchoring protein 2, MGC9414, mitochondrial A kinase PPKA anchor protein 10, PRKA10a kinase anchor protein 10, mitochondrial, protein kinase A anchoring protein 10, Protein kinase A-anchoring protein 10

Entrez Gene IDs

11216 (Human)

Gene Symbol

AKAP10

OMIM

152430 (Human)

UniProt

NP_009133 (Human)

Additional AKAP10 Products

Product Documents for AKAP10 Antibody (8C10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AKAP10 Antibody (8C10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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