AKAP10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-94882

Novus Biologicals, part of Bio-Techne
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NB100-94882
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Key Product Details

Species Reactivity

Validated:

Rat

Predicted:

Bovine (100%), Canine (100%), Human (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all AKAP10 Antibodies »

Product Summary for AKAP10 Antibody

Immunogen

Peptide with sequence C-RKVKGKEQEKTSD corresponding to internal region according to NP_009133.2.

Epitope

C-RKVKGKEQEKTSD

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for AKAP10 Antibody

Western Blot: AKAP10 Antibody [NB100-94882]

Western Blot: AKAP10 Antibody [NB100-94882]

Western Blot: AKAP10 Antibody [NB100-94882] - Analysis of AKAP10 using this antibody at 1 ug/ml in Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for AKAP10 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 74 kDa band observed in rat brain lysates (calculated MW of 73.8 kDa band according to human NP_009133.2 and according to rat NP_001108078.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AKAP10

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction. [provided by RefSeq]

Alternate Names

A kinase (PRKA) anchor protein 10, AKAP-10, A-kinase anchor protein 10, mitochondrial, D-AKAP2, D-AKAP-2, Dual specificity A kinase-anchoring protein 2, dual-specificity A-kinase anchoring protein 2, MGC9414, mitochondrial A kinase PPKA anchor protein 10, PRKA10a kinase anchor protein 10, mitochondrial, protein kinase A anchoring protein 10, Protein kinase A-anchoring protein 10

Entrez Gene IDs

11216 (Human); 56697 (Mouse); 360540 (Rat)

Gene Symbol

AKAP10

UniProt

NP_009133.2 (Human)

Additional AKAP10 Products

Product Documents for AKAP10 Antibody

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Certificate of Analysis

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Product Specific Notices for AKAP10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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