AKAP12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38786

Novus Biologicals, part of Bio-Techne
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NBP2-38786
NBP2-38786-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AKAP12 Antibodies »

Product Summary for AKAP12 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SKDMSEASEKTMTVEVEGSTVNDQQLEEVVLPSEEEGGGAGTKSVPEDDGHALLAERIEKSLVEPKEDEKGDDVDDPENQNSALADTDASGGLTKESPDTNGPKQKEKEDAQEVELQEGKVHSESDKAITPQAQEELQKQERESAKS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AKAP12 Antibody

Immunocytochemistry/ Immunofluorescence: AKAP12 Antibody [NBP2-38786]

Immunocytochemistry/ Immunofluorescence: AKAP12 Antibody [NBP2-38786]

Immunocytochemistry/Immunofluorescence: AKAP12 Antibody [NBP2-38786] - Staining of human cell line U-251 MG shows localization to plasma membrane and cytosol.
Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP2-38786]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP2-38786]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP2-38786] - Staining in human testis and skeletal muscle tissues using NBP2-38786 antibody. Corresponding AKAP12 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP2-38786]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP2-38786]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP2-38786] - Staining of human endometrium shows moderate positivity in stromal cells.
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Applications for AKAP12 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AKAP12

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Alternate Names

A kinase (PRKA) anchor protein (gravin) 12, A kinase (PRKA) anchor protein 12, AKAP 250, AKAP250250kDa, DKFZp686M0430, DKFZp686O0331, FLJ20945, FLJ97621, Gravin, kinase scaffold protein gravin, Myasthenia gravis autoantigen, myasthenia gravis autoantigen gravin

Gene Symbol

AKAP12

UniProt

Q02952 (Human)

Additional AKAP12 Products

Product Documents for AKAP12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AKAP12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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