Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all AKAP4 Antibodies »

Product Specifications

Immunogen

AKAP4 antibody was raised against a 17 amino acid peptide near the amino terminus of human AKAP4. The immunogen is located within the first 50 amino acids of AKAP4. Amino Acid Squence: KVDLYNPEGQQDQDRK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse: (88%)

Specificity

AKAP4 antibody is human specific. At least three isoforms are known to exist; this antibody will detect the two longest isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AKAP4 Antibody - BSA Free

Western Blot: AKAP4 AntibodyBSA Free [NBP2-81946]

Western Blot: AKAP4 AntibodyBSA Free [NBP2-81946]

Western Blot: AKAP4 Antibody [NBP2-81946] - Analysis of AKAP4 in human testis tissue lysate with AKAP4 antibody at (A) 1 and (B) 2 ug/ml.
AKAP4 Antibody - BSA Free

Immunohistochemistry: AKAP4 Antibody - BSA Free [NBP2-81946] -

Immunohistochemistry: AKAP4 Antibody - BSA Free [NBP2-81946] - Immunohistochemistry of AKAP4 in human testis tissue with AKAP4 antibody at 5 u/ml.
AKAP4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: AKAP4 Antibody - BSA Free [NBP2-81946] -

Immunocytochemistry/ Immunofluorescence: AKAP4 Antibody - BSA Free [NBP2-81946] - Immunofluorescence of AKAP4 in human testis tissue with AKAP4 antibody at 20 u/ml.

Applications for AKAP4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AKAP4

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Alternate Names

A kinase (PRKA) anchor protein 4, AKAP-4, AKAP82AKAP 82, A-kinase anchor protein 4, A-kinase anchor protein 82 kDa, cancer/testis antigen 99, CT99, hAKAP82Fsc1, HIFSC1, Major sperm fibrous sheath protein, p82, PRKA4, protein kinase A anchoring protein 4, Protein kinase A-anchoring protein 4, testis-specific gene HI

Gene Symbol

AKAP4

Additional AKAP4 Products

Product Documents for AKAP4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AKAP4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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