AKAP9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89166

Novus Biologicals, part of Bio-Techne
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NBP1-89166
NBP1-89166-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AKAP9 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KLLEAISETSSQLEHAKVTQTELMRESFRQKQEATESLKCQEELRERLHEESRAREQLAVELSKAEGVIDGYADEKTLFERQIQEKTDIIDRLEQELLCASNRLQELEAEQQQIQEERELLSRQKEAMKAEAGPVEQQLLQETE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AKAP9 Antibody

Immunohistochemistry-Paraffin: AKAP9 Antibody [NBP1-89166]

Immunohistochemistry-Paraffin: AKAP9 Antibody [NBP1-89166]

Immunohistochemistry-Paraffin: AKAP9 Antibody [NBP1-89166] - Analysis in human small intestine and pancreas tissues. Corresponding AKAP9 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: AKAP9 Antibody [NBP1-89166]

Immunocytochemistry/ Immunofluorescence: AKAP9 Antibody [NBP1-89166]

Immunocytochemistry/Immunofluorescence: AKAP9 Antibody [NBP1-89166] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: AKAP9 Antibody [NBP1-89166]

Immunohistochemistry-Paraffin: AKAP9 Antibody [NBP1-89166]

Immunohistochemistry-Paraffin: AKAP9 Antibody [NBP1-89166] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
View 3 more images

Applications for AKAP9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

Reported in scientific literature (PMID 25366663)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AKAP9

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in many isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3. [provided by RefSeq]

Alternate Names

A kinase (PRKA) anchor protein (yotiao) 9, AKAP 350, AKAP 450, AKAP350A-kinase anchor protein 450 kDa, AKAP450A-kinase anchor protein 350 kDa, AKAP-9, Centrosome- and Golgi-localized PKN-associated protein, CG-NAPAKAP 120-like protein, hgAKAP 350, HYPERION, KIAA0803A-kinase anchor protein 9, MU-RMS-40.16A, PRKA9AKAP9-BRAF fusion protein, Protein hyperion, protein kinase A anchoring protein 9, Protein kinase A-anchoring protein 9, Protein yotiao, YOTIAOkinase N-associated protein

Gene Symbol

AKAP9

Additional AKAP9 Products

Product Documents for AKAP9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AKAP9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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