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AKR1A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89121

Novus Biologicals, part of Bio-Techne
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NBP1-89121
NBP1-89121-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for AKR1A1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NPFPKNADGTICYDSTHYKETWKALEALVAKGLVQALGLSNFNSRQIDDILSVASVRPAVLQVECHPYLAQNELIAH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AKR1A1 Antibody

Western Blot: AKR1A1 Antibody [NBP1-89121]

Western Blot: AKR1A1 Antibody [NBP1-89121]

Western Blot: AKR1A1 Antibody [NBP1-89121] - Analysis in control (vector only transfected HEK293T lysate) and AKR1A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: AKR1A1 Antibody [NBP1-89121]

Immunocytochemistry/ Immunofluorescence: AKR1A1 Antibody [NBP1-89121]

Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody [NBP1-89121] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: AKR1A1 Antibody [NBP1-89121]

Immunohistochemistry-Paraffin: AKR1A1 Antibody [NBP1-89121]

Immunohistochemistry-Paraffin: AKR1A1 Antibody [NBP1-89121] - Staining of human pancreas shows cytoplasmic positivity both in exocrine glandular cells and islet cells.

Applications for AKR1A1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AKR1A1

AKR1A1 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Alternative splicing of this gene results in two transcript variants encoding the same protein.

Long Name

Aldo-keto reductase family 1 member A1

Alternate Names

Aldehyde reductase, ALDR1, EC 1.1.1.19, EC 1.1.1.2, EC 1.1.1.20, EC 1.1.1.372, EC 1.1.1.54

Gene Symbol

AKR1A1

Additional AKR1A1 Products

Product Documents for AKR1A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AKR1A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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