ALAD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89157

Novus Biologicals, part of Bio-Techne
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NBP1-89157
NBP1-89157-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ALAD Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VLIFGVPSRVPKDERGSAADSEESPAIEAIHLLRKTFPNLLVACDVCLCPYTSHGHCGLLSENGAFRAEESRQRLAEVALAYA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALAD Antibody

Western Blot: ALAD Antibody [NBP1-89157]

Western Blot: ALAD Antibody [NBP1-89157]

Western Blot: ALAD Antibody [NBP1-89157] - Analysis in human cell line HepG2.
Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89157]

Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89157]

Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89157] - Staining of human stomach shows distinct cytoplasmic positivity in glandular cells.
ALAD Antibody Immunohistochemistry-Paraffin: ALAD Antibody Antibody [NBP1-89157]

Immunohistochemistry-Paraffin: ALAD Antibody Antibody [NBP1-89157]

Staining of human kidney, liver, pancreas and skeletal muscle using NBP1-89157 (A) shows similar protein distribution across tissues to independent antibody NBP1-89158 (B).

Applications for ALAD Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALAD

The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. [provided by RefSeq]

Alternate Names

ALADHEC 4.2.1.24, aminolevulinate dehydratase, aminolevulinate, delta-, dehydratase, delta-aminolevulinic acid dehydratase, PBGSMGC5057, Porphobilinogen synthase

Entrez Gene IDs

210 (Human)

Gene Symbol

ALAD

UniProt

P13716 (Human)

Additional ALAD Products

Product Documents for ALAD Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ALAD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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