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Alanyl tRNA synthetase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31706

Novus Biologicals, part of Bio-Techne
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NBP1-31706

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Alanyl tRNA synthetase. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

107 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Alanyl tRNA synthetase Antibody

Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706]

Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706]

Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP1-31706]

Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP1-31706]

Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP1-31706] - Paraffin-embedded hepatoma; antibody dilution 1:500.
Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706]

Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706]

Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706] - (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7.5% SDS PAGE diluted at 1:1000

Applications for Alanyl tRNA synthetase Antibody

Application
Recommended Usage

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Alanyl tRNA synthetase

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq]

Alternate Names

alanine tRNA ligase 1, cytoplasmic, Alanine--tRNA ligase, alanyl-tRNA synthetase, alanyl-tRNA synthetase, cytoplasmic, AlaRS, CMT2N, EC 6.1.1, EC 6.1.1.7, Renal carcinoma antigen NY-REN-42

Entrez Gene IDs

16 (Human)

Gene Symbol

AARS1

UniProt

Additional Alanyl tRNA synthetase Products

Product Documents for Alanyl tRNA synthetase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Alanyl tRNA synthetase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.