ALAS2 Antibody (6C1)

Novus Biologicals, part of Bio-Techne | Catalog # H00000212-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6C1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ALAS2 Antibodies »

Product Specifications

Immunogen

ALAS2 (NP_000023, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVTAAMLLQCCPVLARGPTSLLGKVVKTHQFLFGIGRCPILATQGPNCSQIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFK

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

ALAS2 - aminolevulinate, delta-, synthase 2 (sideroblastic/hypochromic anemia)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ALAS2 Antibody (6C1)

Western Blot: ALAS2 Antibody (6C1) [H00000212-M01]

Western Blot: ALAS2 Antibody (6C1) [H00000212-M01]

Western Blot: ALAS2 Antibody (6C1) [H00000212-M01] - Analysis of ALAS2 expression in transfected 293T cell line by ALAS2 monoclonal antibody (M01), clone 6C1.Lane 1: ALAS2 transfected lysate(64 KDa).Lane 2: Non-transfected lysate.
Western Blot: ALAS2 Antibody (6C1) [H00000212-M01]

Western Blot: ALAS2 Antibody (6C1) [H00000212-M01]

Western Blot: ALAS2 Antibody (6C1) [H00000212-M01] - ALAS2 monoclonal antibody (M01), clone 6C1. Analysis of ALAS2 expression in Raw 264.7.
ELISA: ALAS2 Antibody (6C1) [H00000212-M01]

ELISA: ALAS2 Antibody (6C1) [H00000212-M01]

ELISA: ALAS2 Antibody (6C1) [H00000212-M01] - Detection limit for recombinant GST tagged ALAS2 is approximately 0.1ng/ml as a capture antibody.

Applications for ALAS2 Antibody (6C1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ALAS2

The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified.

Alternate Names

5-aminolevulinic acid synthase 2,5-aminolevulinate synthase, erythroid-specific, mitochondrial, ALASE, ALAS-E, aminolevulinate, delta-, synthase 2, aminolevulinate, delta-, synthase 2 (sideroblastic/hypochromic anemia), ANH1, ASBXLDPP, Delta-ALA synthase 2, delta-ALA synthetase, Delta-aminolevulinate synthase 2, EC 2.3.1.37, FLJ93603, XLSA

Entrez Gene IDs

212 (Human)

Gene Symbol

ALAS2

OMIM

301300 (Human)

UniProt

NP_000023 (Human)

Additional ALAS2 Products

Product Documents for ALAS2 Antibody (6C1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ALAS2 Antibody (6C1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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