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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Full length protein (Mouse)

Specificity

By immunoelectrophoresis, the antiserum reacts specifically with mouse Albumin. No reactivity was detected against other mouse serum proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Albumin Antibody - BSA Free

Western Blot: Albumin AntibodyBSA Free [NB600-41532]

Western Blot: Albumin AntibodyBSA Free [NB600-41532]

Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Albumin Antibody - BSA Free [NB600-41532]

Immunocytochemistry/ Immunofluorescence: Albumin Antibody - BSA Free [NB600-41532]

Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY license.
Albumin Antibody - BSA Free

Western Blot: Albumin Antibody - BSA Free [NB600-41532] -

Western Blot: Albumin Antibody - BSA Free [NB600-41532] - Primary cell characterization.As per the materials & methods, tumors were isolated from mice & cultured. A, Primary murine hepatocytes were used as controls (Mu). Western blot revealed the primary cultures of APN KO A51, A48, A50 & A52 & WT C53, C54 & C55 cells to express p-mTOR, mTOR, p-Src, Src, PCNA, albumin & AFP. B, Immunofluorescence for K8 & pan-cytokeratin (PK) confirmed that A52 cells were epithelial (Scale bar 20 μM). C, Table showing in vitro & in vivo growth characteristics of WT & APN KO primary cultures. Scale of growth (+ to ++++). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30794695), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Albumin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:2000

Immunohistochemistry

1:200 - 1:2000

Immunohistochemistry-Paraffin

1:200 - 1:2000

Western Blot

1:1000 - 1:30000
Application Notes
Use in IHC-Fr reported in scientific literature (PMID: 33277474). In ELISA, for coating plates use antibody at 1:100 - 1:500. Albumin antibody validated for WB from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 2 using NB600-41532 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Albumin

Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Alternate Names

ALB

Entrez Gene IDs

11657 (Mouse)

Gene Symbol

ALB

UniProt

Additional Albumin Products

Product Documents for Albumin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Albumin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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