Albumin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-41532
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Full length protein (Mouse)
Specificity
By immunoelectrophoresis, the antiserum reacts specifically with mouse Albumin. No reactivity was detected against other mouse serum proteins.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Albumin Antibody - BSA Free
Western Blot: Albumin AntibodyBSA Free [NB600-41532]
Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. WB image submitted by a verified customer review.Immunocytochemistry/ Immunofluorescence: Albumin Antibody - BSA Free [NB600-41532]
Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY license.Western Blot: Albumin Antibody - BSA Free [NB600-41532] -
Western Blot: Albumin Antibody - BSA Free [NB600-41532] - Primary cell characterization.As per the materials & methods, tumors were isolated from mice & cultured. A, Primary murine hepatocytes were used as controls (Mu). Western blot revealed the primary cultures of APN KO A51, A48, A50 & A52 & WT C53, C54 & C55 cells to express p-mTOR, mTOR, p-Src, Src, PCNA, albumin & AFP. B, Immunofluorescence for K8 & pan-cytokeratin (PK) confirmed that A52 cells were epithelial (Scale bar 20 μM). C, Table showing in vitro & in vivo growth characteristics of WT & APN KO primary cultures. Scale of growth (+ to ++++). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30794695), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Albumin Antibody - BSA Free
Application
Recommended Usage
ELISA
1:1000 - 1:30000
Immunocytochemistry/ Immunofluorescence
1:200 - 1:2000
Immunohistochemistry
1:200 - 1:2000
Immunohistochemistry-Paraffin
1:200 - 1:2000
Western Blot
1:1000 - 1:30000
Application Notes
Use in IHC-Fr reported in scientific literature (PMID: 33277474). In ELISA, for coating plates use antibody at 1:100 - 1:500. Albumin antibody validated for WB from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 2 using NB600-41532 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Albumin
Additional Albumin Products
Product Documents for Albumin Antibody - BSA Free
Product Specific Notices for Albumin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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