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Albumin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32458

Novus Biologicals, part of Bio-Techne
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NBP1-32458

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Feline

Cited:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vitro assay, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Albumin. The exact sequence is proprietary.

Reactivity Notes

Cat (100%), Rabbit (82%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Albumin Antibody

Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458]

Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458]

Immunocytochemistry/Immunofluorescence: Albumin Antibody [NBP1-32458] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: Albumin protein stained by Albumin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458]

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458]

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Mouse liver. Albumin stained by Albumin antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Albumin Antibody [NBP1-32458]

Western Blot: Albumin Antibody [NBP1-32458]

Western Blot: Albumin Antibody [NBP1-32458] - Human plasma (1 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for Albumin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

In vitro assay

Assay dependent

Western Blot

1:500-1:100000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Albumin

Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Alternate Names

ALB

Gene Symbol

ALB

Additional Albumin Products

Product Documents for Albumin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Albumin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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