alcohol dehydrogenase 5 Antibody (2D11)

Novus Biologicals, part of Bio-Techne | Catalog # H00000128-M12-100ug

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
H00000128-M12-100ug
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2D11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all alcohol dehydrogenase 5 Antibodies »

Product Specifications

Immunogen

ADH5 (AAH14665, 1 a.a. ~ 374 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGGEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for alcohol dehydrogenase 5 Antibody (2D11)

Western Blot: alcohol dehydrogenase 5 Antibody (2D11) [H00000128-M12-100ug]

Western Blot: alcohol dehydrogenase 5 Antibody (2D11) [H00000128-M12-100ug]

Western Blot: alcohol dehydrogenase 5 Antibody (2D11) [H00000128-M12-100ug] - Analysis of ADH5 expression in transfected 293T cell line by ADH5 monoclonal antibody (M12), clone 2D11. Lane 1: ADH5 transfected lysate (Predicted MW: 39.7 KDa).Lane 2: Non-transfected lysate.
Western Blot: alcohol dehydrogenase 5 Antibody (2D11) [H00000128-M12-100ug]

Western Blot: alcohol dehydrogenase 5 Antibody (2D11) [H00000128-M12-100ug]

Western Blot: alcohol dehydrogenase 5 Antibody (2D11) [H00000128-M12-100ug] - ADH5 monoclonal antibody (M12), clone 2D11. Western Blot analysis of ADH5 expression in IMR-32.
ELISA: alcohol dehydrogenase 5 Antibody (2D11) [H00000128-M12-100ug]

ELISA: alcohol dehydrogenase 5 Antibody (2D11) [H00000128-M12-100ug]

ELISA: alcohol dehydrogenase 5 Antibody (2D11) [H00000128-M12-100ug] - Detection limit for recombinant GST tagged ADH5 is 0.1 ng/ml as a capture antibody.

Applications for alcohol dehydrogenase 5 Antibody (2D11)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alcohol dehydrogenase 5

The ADH5 gene encodes glutathione dependent formaldehyde dehydrogenase or class III alcohol dehydrogenase chi subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, retinol, ethanol and other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class III alcohol dehydrogenase is a homodimer composed of 2 chi subunits. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long chain primary alcohols and for oxidation of S hydroxymethyl glutathione, a spontaneous adduct between formaldehyde and glutathione. The enzyme is an important component of cellular metabolism for the elimination of formaldehyde, which is a potent irritant and sensitizing agent that causes rhinitis, lacrymation, contact dermatitis and pharyngitis.

Alternate Names

ADH-3, ADHXEC 1.1.1.1, alcohol dehydrogenase (class III), chi polypeptide, Alcohol dehydrogenase 5, alcohol dehydrogenase 5 (class III), chi polypeptide, Alcohol dehydrogenase class chi chain, alcohol dehydrogenase class-3, Alcohol dehydrogenase class-III, EC 1.1.1, EC 1.1.1.-, EC 1.1.1.284, FALDH, FDHGSNOR, formaldehyde dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH, S-(hydroxymethyl)glutathione dehydrogenase

Entrez Gene IDs

128 (Human)

Gene Symbol

ADH5

OMIM

103710 (Human)

UniProt

AAH14665 (Human)

Additional alcohol dehydrogenase 5 Products

Product Documents for alcohol dehydrogenase 5 Antibody (2D11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for alcohol dehydrogenase 5 Antibody (2D11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.