alcohol dehydrogenase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-912
![Western Blot: alcohol dehydrogenase Antibody [NB600-912]](https://resources.bio-techne.com/images/products/alcohol-dehydrogenase-Antibody-Western-Blot-NB600-912-img0006.jpg "Western Blot: alcohol dehydrogenase Antibody [NB600-912]")
Key Product Details
Species Reactivity
Validated:
Yeast
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Summary for alcohol dehydrogenase Antibody
Immunogen
alcohol dehydrogenase [Yeast] (Uniprot: P00330)
Reactivity Notes
Cross reactivity against alcohol dehydrogenase from other sources may occur but have not been specifically determined.
Localization
Cytoplasmic
Specificity
Recognises Alcohol Dehydrogenase. Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for alcohol dehydrogenase Antibody
Western Blot: alcohol dehydrogenase Antibody [NB600-912]
Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Analysis using Azide/BSA FREE version of NB600-912. Detection of yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates estimated molecular weights consistent with monomer, dimer and tetramer present in this preparation. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 2 h followed by detection using 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at room temperature.![Western Blot: alcohol dehydrogenase Antibody [NB600-912]](https://resources.bio-techne.com/images/products/alcohol-dehydrogenase-Antibody-Western-Blot-NB600-912-img0004.jpg "Western Blot: alcohol dehydrogenase Antibody [NB600-912]")
Western Blot: alcohol dehydrogenase Antibody [NB600-912]
Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Lane 1: Alcohol Dehydrogenase. Load: 50ng per lane. Primary antibody: Alcohol Dehydrogenase Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 37kDa, 45kDa.![Western Blot: alcohol dehydrogenase Antibody [NB600-912]](https://resources.bio-techne.com/images/products/alcohol-dehydrogenase-Antibody-Western-Blot-NB600-912-img0005.jpg "Western Blot: alcohol dehydrogenase Antibody [NB600-912]")
Western Blot: alcohol dehydrogenase Antibody [NB600-912]
Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Analysis using Biotin conjugate of NB600-912. Lane 1: Alcohol Dehydrogenase (yeast). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alcohol Dehydrogenase (Yeast) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Streptavidin Antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 37 kDa, 45 kDa for Alcohol Dehydrogenase (yeast).Applications for alcohol dehydrogenase Antibody
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunoprecipitation
1:100
Western Blot
1:500-1:2000
Application Notes
This product has been tested by western blot and is suitable for use in ELISA and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: alcohol dehydrogenase
Alternate Names
ADH, alpha subunit, ADH1alcohol dehydrogenase 1A, alcohol dehydrogenase 1 (class I), alpha polypeptide, alcohol dehydrogenase 1A (class I), alpha polypeptide, Alcohol dehydrogenase subunit alpha, aldehyde reductase, EC 1.1.1, EC 1.1.1.1
Gene Symbol
ADH1A
UniProt
Additional alcohol dehydrogenase Products
Product Documents for alcohol dehydrogenase Antibody
Product Specific Notices for alcohol dehydrogenase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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