Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-787
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Peptide ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-EVKTVTVKISQKNS corresponding to C-Terminus according to NP_000680.2.
Specificity
This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design, and with ALDH2 (GeneID 217) with two residues difference from this design.
Marker
Astrocyte Marker
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787]](https://resources.bio-techne.com/images/products/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NB100-787-img0001.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787]")
Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787]
Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787] - Staining of human (A), mouse (B) and rat (C) liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787] -
Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Applications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Peptide ELISA
detection limit 1:1000
Western Blot
0.1-0.3 ug/ml
Application Notes
WB: Approx 55 kDa band observed in human, mouse and rat liver lysates (calculated MW of 54.9 kDa according to NP_000680.2). IHC: successful usage on mouse Peyer's patches is reported in scientific literature (PMID: 15485630).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1
Alternate Names
ALDC, Aldehyde Dehydrogenase 1A1, ALDH-E1, ALDH1A1, PUMB1, RALDH1
Gene Symbol
ALDH1A1
UniProt
Additional Aldehyde Dehydrogenase 1-A1/ALDH1A1 Products
- All Products for Aldehyde Dehydrogenase 1-A1/ALDH1A1
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 cDNA Clones
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 ELISA Kits
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 Lysates
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 Primary Antibodies
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 Proteins and Enzymes
Product Documents for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody
Product Specific Notices for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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