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Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-914

Novus Biologicals, part of Bio-Techne
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NB600-914-100ug

Key Product Details

Species Reactivity

Yeast

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Aldehyde Dehydrogenase 1-A1/ALDH1A1 1-A1/ALDH1A1 [Yeast] (Uniprot: P47771)

Reactivity Notes

Cross reactivity against Aldehyde Dehydrogenase 1-A1/ALDH1A1 from other tissues and species may occur but have not been specifically determined.

Specificity

Cross reactivity against Aldehyde Dehydrogenase 1-A1/ALDH1A1 from other tissues and species may occur but have not been specifically determined.

Marker

Astrocyte Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB600-914]

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB600-914]

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB600-914] - Western Blot Results of Rabbit Aldehyde Dehydrogenase 1-A1/ALDH1A1 1-A1/ALDH1A1 antibody. Lane 1: Molecular Weight Marker. Lane 2: Aldehyde Dehydrogenase 1-A1/ALDH1A1.Primary Antibody: Anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG at 1:40,000 for 30 mins at RT. Block: Blocking buffer for 30 min at RT. Expect MW: ~55kDa.
Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Western Blot Results of Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody. Lane 1: Molecular Weight Marker. Lane 2: Aldehyde Dehydrogenase. Primary Antibody: Anti-Aldehyde Dehydrogenase at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG

Applications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunoprecipitation

1:100

Western Blot

1:500-1:2000
Application Notes
This product has been tested by western blot. Assays should be optimized by the end user for specific conditions for reactivity.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1

Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.

Alternate Names

ALDC, Aldehyde Dehydrogenase 1A1, ALDH-E1, ALDH1A1, PUMB1, RALDH1

Gene Symbol

ALDH1A1

UniProt

Additional Aldehyde Dehydrogenase 1-A1/ALDH1A1 Products

Product Documents for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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