Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-914
Key Product Details
Species Reactivity
Validated:
Yeast
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Summary for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody
Immunogen
Aldehyde Dehydrogenase 1-A1/ALDH1A1 1-A1/ALDH1A1 [Yeast] (Uniprot: P47771)
Reactivity Notes
Cross reactivity against Aldehyde Dehydrogenase 1-A1/ALDH1A1 from other tissues and species may occur but have not been specifically determined.
Specificity
Cross reactivity against Aldehyde Dehydrogenase 1-A1/ALDH1A1 from other tissues and species may occur but have not been specifically determined.
Marker
Astrocyte Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody
Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB600-914]
Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB600-914] - Western Blot Results of Rabbit Aldehyde Dehydrogenase 1-A1/ALDH1A1 1-A1/ALDH1A1 antibody. Lane 1: Molecular Weight Marker. Lane 2: Aldehyde Dehydrogenase 1-A1/ALDH1A1.Primary Antibody: Anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG at 1:40,000 for 30 mins at RT. Block: Blocking buffer for 30 min at RT. Expect MW: ~55kDa.Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody
Western Blot Results of Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody. Lane 1: Molecular Weight Marker. Lane 2: Aldehyde Dehydrogenase. Primary Antibody: Anti-Aldehyde Dehydrogenase at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgGApplications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunoprecipitation
1:100
Western Blot
1:500-1:2000
Application Notes
This product has been tested by western blot. Assays should be optimized by the end user for specific conditions for reactivity.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1
Alternate Names
ALDC, Aldehyde Dehydrogenase 1A1, ALDH-E1, ALDH1A1, PUMB1, RALDH1
Gene Symbol
ALDH1A1
UniProt
Additional Aldehyde Dehydrogenase 1-A1/ALDH1A1 Products
- All Products for Aldehyde Dehydrogenase 1-A1/ALDH1A1
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 cDNA Clones
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 ELISA Kits
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 Lysates
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 Primary Antibodies
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 Proteins and Enzymes
Product Documents for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody
Product Specific Notices for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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