Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52052

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RYPPSPAKMTQH corresponding to C-Terminus according to NP_000682.3.

Epitope

C-RYPPSPAKMTQH

Specificity

Reported variants represent identical protein: NP_001128639.1, NP_000682.3, NP_001128640.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody

Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP1-52052]

Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP1-52052]

Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP1-52052] - (0.01ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP1-52052]

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP1-52052]

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP1-52052] - Staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Immunohistochemistry-Paraffin

3-5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in mouse eye lysates (calculated MW of 50.5 kDa band according to mouse NP_031462.2). IHC-P: Human Lung shows strong staining of the respiratory epithelium of the tracheal wall.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aldehyde Dehydrogenase 3-A1/ALDH3A1

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Alternate Names

Aldehyde Dehydrogenase 3A1, ALDH3A1, ALDHIII

Entrez Gene IDs

218 (Human)

Gene Symbol

ALDH3A1

UniProt

NP_000682.3 (Human)

Additional Aldehyde Dehydrogenase 3-A1/ALDH3A1 Products

Product Documents for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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