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Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15340

Novus Biologicals, part of Bio-Techne
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NBP2-15340

Key Product Details

Species Reactivity

Validated:

Mouse, Bovine, Canine

Predicted:

Human (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aldehyde Dehydrogenase 3-A1/ALDH3A1. The exact sequence is proprietary.

Reactivity Notes

Rat (83%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody

Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340]

Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340]

Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340] - Whole cell extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH3A1 antibody [N1C2] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Frozen: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340]

Immunohistochemistry-Frozen: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340]

Immunohistochemistry-Frozen: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340] - Analysis of frozen bovine cornea section, using ALDH3A1 antibody at 1:100 dilution.
Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody

Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340] -Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH3A1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340] -Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH3A1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Aldehyde Dehydrogenase 3-A1/ALDH3A1

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Alternate Names

Aldehyde Dehydrogenase 3A1, ALDH3A1, ALDHIII

Gene Symbol

ALDH3A1

UniProt

Additional Aldehyde Dehydrogenase 3-A1/ALDH3A1 Products

Product Documents for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.