ALDH1A2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06323

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ALDH1A2 Antibodies »

Product Summary for ALDH1A2 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human ALDH1A2 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ALDH1A2 Antibody

Western Blot: ALDH1A2 Antibody [NBP3-06323]

Western Blot: ALDH1A2 Antibody [NBP3-06323]

Western Blot: ALDH1A2 Antibody [NBP3-06323] - Anti-ALDH1A2 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:57 kDaObserved band size:55 kDa
Immunoprecipitation: ALDH1A2 Antibody [NBP3-06323]

Immunoprecipitation: ALDH1A2 Antibody [NBP3-06323]

Immunoprecipitation: ALDH1A2 Antibody [NBP3-06323] - ALDH1A2 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate4 uL anti-ALDH1A2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-ALDH1A2 rabbit polyclonal antibody, at 1:100 dilution.Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 57 kDaObserved band size :55 kDa

Applications for ALDH1A2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH1A2

ALDH1A2 belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Aldehyde Dehydrogenase 1 Family, Member A2

Alternate Names

ALDH 1A2, RALDH, RALDH2

Gene Symbol

ALDH1A2

Additional ALDH1A2 Products

Product Documents for ALDH1A2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ALDH1A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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