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ALDH1A3 Antibody (OTI4E8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46510

Novus Biologicals, part of Bio-Techne
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NBP2-46510

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4E8

Concentration

0.8 mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-100 and 413-512 of human ALDH1A3 (NP_000648) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

55.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALDH1A3 Antibody (OTI4E8)

Western Blot: ALDH1A3 Antibody (OTI4E8) [NBP2-46510]

Western Blot: ALDH1A3 Antibody (OTI4E8) [NBP2-46510]

Western Blot: ALDH1A3 Antibody (4E8) [NBP2-46510] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: ALDH1A3 Antibody (OTI4E8) [NBP2-46510]

Immunohistochemistry: ALDH1A3 Antibody (OTI4E8) [NBP2-46510]

Immunohistochemistry: ALDH1A3 Antibody (4E8) [NBP2-46510] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Flow Cytometry: ALDH1A3 Antibody (OTI4E8) [NBP2-46510]

Flow Cytometry: ALDH1A3 Antibody (OTI4E8) [NBP2-46510]

Flow Cytometry: ALDH1A3 Antibody (4E8) [NBP2-46510] - Analysis of Jurkat cells, using ALDH1A3 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Applications for ALDH1A3 Antibody (OTI4E8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.8 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aldehyde Dehydrogenase 1-A3/ALDH1A3

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq].

Long Name

Aldehyde Dehydrogenase 1 Family Member A3

Alternate Names

ALDH1A3, ALDH6, MCOP8, RALDH3

Gene Symbol

ALDH1A3

Additional Aldehyde Dehydrogenase 1-A3/ALDH1A3 Products

Product Documents for ALDH1A3 Antibody (OTI4E8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ALDH1A3 Antibody (OTI4E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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