ALDH1A3 Antibody - BSA Free

Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - IL25 Deficiency Induced loss of CRC stemness. The expression levels of CSC markers, namely, DCLK1, ALDH1 (NBP2-15339), and CD44, were examined in tumor tissues by Western blotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35359953/) licensed under a CC-BY license.

Western Blot: ALDH1A3 AntibodyBSA Free [NBP2-15339]

Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: ALDH1A3 AntibodyBSA Free [NBP2-15339]

Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:2000.

Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Blue: Hoechst 33342 staining.

Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Human breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T. Human breast cancer cells were transduced with human Aldh1a3 and probed with antibody at 1:500 overnight at 4C followed by counterstaining with secondary and DAPI. Strong signal observed compared to knockout controls. ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Frozen: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunohistochemistry-Frozen: ALDH1A3 Antibody - BSA Free [NBP2-15339] - 8-week old mouse pancreas cryosections with beta cell dedifferentiation properties were stained with ALDH1A3 (1:500 dilution) and Insulin (1:1000 dilution) antibodies. Blue arrows indicate insulin+Aldh1A3+ cells. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Rat prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] -

Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Palbociclib induces a potential transition of PN GSCs to MES GSCs(A) qPCR analysis of markers of PN & MES subtypes in G44 & G559 treated with the indicated doses of palbociclib for 5 days. n = 3. Differences were analyzed between the control & the palbociclib-treated groups. NS: not statistically significant; #p < 0.05; *p < 0.01; **p < 0.001. (B) qPCR & immunoblot analysis of ALDH1A3 in three independent PN GSC lines that were treated with 10 nM of palbociclib for 5 days. n = 3. Differences were analyzed between the control & the palbociclib-treated groups. NS: not statistically significant; #p < 0.05; *p < 0.01; **p < 0.001. (C) Cell proliferation analysis of three independent PN GSC lines that were treated with palbociclib (10 nM), DEAB (25 μM), or both for 5 days. The cells were cultured on a laminin (10 μg/ml in poly ornithine)-coated 96-well plate. Cell number was determined by CyQUANT Direct Cell Proliferation assay. n = 3. *p < 0.05, comparison between palbociclib or DEAB treatment with the vehicle control; #p < 0.05, comparison between combination group with palbociclib treatment. G44, CDI = 0.79; G559, CDI = 0.565; G816, CDI = 0.677. Shown are representative data of three independent experiments with similar results. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.19429), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] -

Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Markers of dedifferentiation are present in islets of db/db, but not weight-matched WD-fed mice. (a) Immunofluorescent analysis of islets from weight-matched WD-fed mice (top row) & db/db mice (bottom row) showing Nkx6.1 (red), insulin (green), & DAPI (blue). In the merged image, note the loss of double-positive nuclei (DAPI plus Nkx6.1; purple color) in the db/db mice but not in the WD-fed mice. (b) Expression of the Aldh1a3 gene in islets isolated from mice fed a WD for 4 or 12 weeks normalized to mice fed a control diet compared with db/db mice at 8 weeks of age (normalized to lean db/+ controls). ∗∗∗p < 0.001 versus both WD groups by one-way ANOVA. (c) Staining for Aldh1a3 protein (red) & insulin (green) in islets from weight-matched WD-fed mice (20 weeks on diet) versus db/db mice (14 weeks of age). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29038790), licensed under a CC-BY license. Not internally tested by Novus Biologicals.