ALDH2 Antibody (1E5)

Novus Biologicals, part of Bio-Techne | Catalog # H00000217-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ALDH2 Antibodies »

Product Specifications

Immunogen

ALDH2 (AAH02967, 408 a.a. ~ 517 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWVNCYDVFGAQSPFGGYKMSGSGRELGEYGLQAYTEVKTVTVKVPQKNS

Specificity

ALDH2 - aldehyde dehydrogenase 2 family (mitochondrial)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ALDH2 Antibody (1E5)

Western Blot: ALDH2 Antibody (1E5) [H00000217-M01]

Western Blot: ALDH2 Antibody (1E5) [H00000217-M01]

Western Blot: ALDH2 Antibody (1E5) [H00000217-M01] - Analysis of ALDH2 expression in transfected 293T cell line by ALDH2 monoclonal antibody (M01), clone 1E5.Lane 1: ALDH2 transfected lysate(56.4 KDa).Lane 2: Non-transfected lysate.
Western Blot: ALDH2 Antibody (1E5) [H00000217-M01]

Western Blot: ALDH2 Antibody (1E5) [H00000217-M01]

Western Blot: ALDH2 Antibody (1E5) [H00000217-M01] - ALDH2 monoclonal antibody (M01), clone 1E5 Analysis of ALDH2 expression in MCF-7.
ELISA: ALDH2 Antibody (1E5) [H00000217-M01]

ELISA: ALDH2 Antibody (1E5) [H00000217-M01]

ELISA: ALDH2 Antibody (1E5) [H00000217-M01] - Detection limit for recombinant GST tagged ALDH2 is approximately 0.3ng/ml as a capture antibody.

Applications for ALDH2 Antibody (1E5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ALDH2

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix.

Long Name

Aldehyde Dehydrogenase 2 Family Member

Alternate Names

ALDHI, ALDM

Entrez Gene IDs

217 (Human)

Gene Symbol

ALDH2

OMIM

100650 (Human)

UniProt

AAH02967 (Human)

Additional ALDH2 Products

Product Documents for ALDH2 Antibody (1E5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ALDH2 Antibody (1E5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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