ALDH5A1 Antibody (17F5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59409

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 17F5

Format

BSA Free

Concentration

1 mg/ml

View all ALDH5A1 Antibodies »

Product Specifications

Immunogen

Recombinant human ALDH5A1 (48-535aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ALDH5A1 Antibody (17F5) - BSA Free

Western Blot: ALDH5A1 Antibody (17F5)BSA Free [NBP2-59409]

Western Blot: ALDH5A1 Antibody (17F5)BSA Free [NBP2-59409]

Western Blot: ALDH5A1 Antibody (17F5) [NBP2-59409] - Figure c: Downregulation of the ALDH5A1 protein product SSADH in TG1-miR. Western blot analysis. SSADH MW, 57 kDa; Actin MW, 42 kDa. Mean +/- SD, n = 3 independent biological samples. Figure d: Decreased ALDH5A1 mRNA levels in TG1-miR compared to TG1. Q-PCR assays. Mean +/- SD, n = 3 independent biological samples. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-016-1659-5), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: ALDH5A1 Antibody (17F5) - BSA Free [NBP2-59409]

Immunocytochemistry/ Immunofluorescence: ALDH5A1 Antibody (17F5) - BSA Free [NBP2-59409]

Immunocytochemistry/Immunofluorescence: ALDH5A1 Antibody (17F5) [NBP2-59409] - Analysis of ALDH5A1 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDH5A1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Immunohistochemistry: ALDH5A1 Antibody (17F5) - BSA Free [NBP2-59409]

Immunohistochemistry: ALDH5A1 Antibody (17F5) - BSA Free [NBP2-59409]

Immunohistochemistry: ALDH5A1 Antibody (17F5) [NBP2-59409] - SSADH immunoreactive cells are enriched in proliferative/non-differentiated GBM territories (PHIGH/D-) and rare in non-proliferative/differentiated (PLOW/D+) tumor territories of patients' GBM, as revealed by immunohistochemical staining. HES: hematoxylin and eosin staining. Scale bar 100 um. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-016-1659-5), licensed under a CC-BY license.
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Applications for ALDH5A1 Antibody (17F5) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH5A1

ALDH5A1 belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

aldehyde dehydrogenase 5 family, member A1, EC 1.2.1, EC 1.2.1.24, NAD(+)-dependent succinic semialdehyde dehydrogenase, SSADHmitochondrial

Gene Symbol

ALDH5A1

Additional ALDH5A1 Products

Product Documents for ALDH5A1 Antibody (17F5) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ALDH5A1 Antibody (17F5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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