ALDH7A1 Antibody (101)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89926

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-89926-100ul
NBP2-89926-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 101

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ALDH7A1 Antibodies »

Product Summary for ALDH7A1 Antibody (101)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ALDH7A1 (Uniprot#: P49419-2; Ser2-Gln511).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ALDH7A1 Antibody (101)

Immunocytochemistry/ Immunofluorescence: ALDH7A1 Antibody (101) [NBP2-89926]

Immunocytochemistry/ Immunofluorescence: ALDH7A1 Antibody (101) [NBP2-89926]

Immunocytochemistry/Immunofluorescence: ALDH7A1 Antibody (101) [NBP2-89926] - Staining of Human ALDH7A1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ALDH7A1 monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Flow Cytometry: ALDH7A1 Antibody (101) [NBP2-89926]

Flow Cytometry: ALDH7A1 Antibody (101) [NBP2-89926]

Flow Cytometry: ALDH7A1 Antibody (101) [NBP2-89926] - Analysis of Human ALDH7A1 expression on HepG2 cells. The cells were treated, stained with purified anti-Human ALDH7A1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for ALDH7A1 Antibody (101)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH7A1

Antiquitin (Aldh7a1) is an evolutionarily conserved protein believed to play a role in the regulation of cellular turgor. Based on sequence analysis, this protein is classified as a member of the aldehyde dehydrogenase superfamily (1). Analysis of the amount of mRNA in various rat and human tissues indicates that the largest amounts are found in rat kidney and liver and in cultured human hepatoma cells. Only minimal amounts were detected in human peripheral blood leukocytes, rat lung, or cultured human fibroblasts (2). The plant homolog of ATQ1 is thought to be involved in regulating turgor pressure, a function that also would be essential for cells of the mammalian cochlea. Northern blots of 13 human fetal tissues show antiquitin to be highly expressed in cochlea, ovary, eye, heart, and kidney (3).

Alternate Names

aldehyde dehydrogenase 7 family, member A1, Aldehyde dehydrogenase family 7 member A1,26g turgor protein homolog, Alpha-AASA dehydrogenase, ATQ1Antiquitin-1, Betaine aldehyde dehydrogenase, Delta1-piperideine-6-carboxylate dehydrogenase, EC 1.2.1, EC 1.2.1.3, EC 1.2.1.31, EC 1.2.1.8, EPDalpha-aminoadipic semialdehyde dehydrogenase, FLJ11738, FLJ92814, P6c dehydrogenase, PDEantiquitin-1

Gene Symbol

ALDH7A1

Additional ALDH7A1 Products

Product Documents for ALDH7A1 Antibody (101)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ALDH7A1 Antibody (101)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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