Aldo-keto Reductase 1B10/AKR1B10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89161

Novus Biologicals, part of Bio-Techne
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NBP1-89161
NBP1-89161-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Aldo-keto Reductase 1B10/AKR1B10 Antibodies »

Product Summary for Aldo-keto Reductase 1B10/AKR1B10 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PTFFERPLVRKAFEKTLKDLKLSYLDVYLIHWPQGFKSGDDLFPKDDKGNAIGGKAT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aldo-keto Reductase 1B10/AKR1B10 Antibody

Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NBP1-89161]

Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NBP1-89161]

Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NBP1-89161] - Analysis in human cell lines A-549 and HEK293 using anti-AKR1B10 antibody. Corresponding AKR1B10 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NBP1-89161]

Immunocytochemistry/ Immunofluorescence: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NBP1-89161]

Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NBP1-89161] - Staining of human cell line A549 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NBP1-89161]

Immunohistochemistry-Paraffin: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NBP1-89161]

Immunohistochemistry-Paraffin: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NBP1-89161] - Staining of human small intestine shows strong cytoplasmic, nuclear and membranous positivity in glandular cells.
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Applications for Aldo-keto Reductase 1B10/AKR1B10 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aldo-keto Reductase 1B10/AKR1B10

AKR1B10 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis.

Long Name

Aldo-keto Reductase Family 1, Member B10 [Aldose Reductase]

Alternate Names

AKR1B10, AKR1B11, AKR1B12, AldoketoReductase 1B10, ARL1, HIS, HSI

Gene Symbol

AKR1B10

Additional Aldo-keto Reductase 1B10/AKR1B10 Products

Product Documents for Aldo-keto Reductase 1B10/AKR1B10 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aldo-keto Reductase 1B10/AKR1B10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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