Aldolase A Antibody (3D9-6F3)

Novus Biologicals, part of Bio-Techne | Catalog # H00000226-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3D9-6F3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Aldolase A Antibodies »

Product Specifications

Immunogen

ALDOA (AAH10660.1, 1 a.a. ~ 364 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFVSNHAY

Specificity

ALDOA - aldolase A, fructose-bisphosphate

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Aldolase A Antibody (3D9-6F3)

Western Blot: Aldolase A Antibody (3D9-6F3) [H00000226-M01]

Western Blot: Aldolase A Antibody (3D9-6F3) [H00000226-M01]

Western Blot: Aldolase A Antibody (3D9-6F3) [H00000226-M01] - Analysis of ALDOA expression in transfected 293T cell line by ALDOA monoclonal antibody (M01), clone 3D9-6F3.Lane 1: ALDOA transfected lysate (Predicted MW: 39.4 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Aldolase A Antibody (3D9-6F3) [H00000226-M01]

Immunocytochemistry/ Immunofluorescence: Aldolase A Antibody (3D9-6F3) [H00000226-M01]

Immunocytochemistry/Immunofluorescence: Aldolase A Antibody (3D9-6F3) [H00000226-M01] - Analysis of monoclonal antibody to ALDOA on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Aldolase A Antibody (3D9-6F3) [H00000226-M01]

Western Blot: Aldolase A Antibody (3D9-6F3) [H00000226-M01]

Western Blot: Aldolase A Antibody (3D9-6F3) [H00000226-M01] - ALDOA monoclonal antibody (M01), clone 3D9-6F3 Analysis of ALDOA expression in IMR-32.
View 1 more images

Applications for Aldolase A Antibody (3D9-6F3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, tissue lysate and recombinant protein for WB. It has been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Aldolase A

This gene product, Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

Alternate Names

ALDA, aldolase A, fructose-bisphosphate, EC 4.1.2.13, fructose-1,6-bisphosphate triosephosphate-lyase, fructose-bisphosphate aldolase A, GSD12, Lung cancer antigen NY-LU-1, MGC10942, MGC17716, MGC17767, Muscle-type aldolase

Entrez Gene IDs

226 (Human)

Gene Symbol

ALDOA

OMIM

103850 (Human)

UniProt

AAH10660 (Human)

Additional Aldolase A Products

Product Documents for Aldolase A Antibody (3D9-6F3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aldolase A Antibody (3D9-6F3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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