Aldolase A Antibody (4B2E5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15381

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15381-100ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4B2E5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Aldolase A Antibodies »

Product Summary for Aldolase A Antibody (4B2E5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Aldolase (P04075). MPYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aldolase A Antibody (4B2E5)

Western Blot: Aldolase A Antibody (4B2E5) [NBP3-15381]

Western Blot: Aldolase A Antibody (4B2E5) [NBP3-15381]

Western Blot: Aldolase A Antibody (4B2E5) [NBP3-15381] - Western blot analysis of extracts of various cell lines, using Aldolase A Rabbit mAb (NBP3-15381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Aldolase A Antibody (4B2E5)

Immunohistochemistry-Paraffin: Aldolase A Antibody (4B2E5) [NBP3-15381] -

Immunohistochemistry-Paraffin: Aldolase A Antibody (4B2E5) [NBP3-15381] -Analysis of paraffin-embedded Human tonsil tissue using ALDOA Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Aldolase A Antibody (4B2E5)

Immunohistochemistry-Paraffin: Aldolase A Antibody (4B2E5) [NBP3-15381] -

Immunohistochemistry-Paraffin: Aldolase A Antibody (4B2E5) [NBP3-15381] -Analysis of paraffin-embedded Mouse heart tissue using ALDOA Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
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Applications for Aldolase A Antibody (4B2E5)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunohistochemistry

1:200 - 1:800

Immunohistochemistry-Paraffin

1:200 - 1:800

Western Blot

1:1000 - 1:6000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aldolase A

Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

Alternate Names

ALDA, aldolase A, fructose-bisphosphate, EC 4.1.2.13, fructose-1,6-bisphosphate triosephosphate-lyase, fructose-bisphosphate aldolase A, GSD12, Lung cancer antigen NY-LU-1, MGC10942, MGC17716, MGC17767, Muscle-type aldolase

Gene Symbol

ALDOA

Additional Aldolase A Products

Product Documents for Aldolase A Antibody (4B2E5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aldolase A Antibody (4B2E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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