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Aldolase A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87488

Novus Biologicals, part of Bio-Techne
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NBP1-87488
NBP1-87488-25ul

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aldolase A Antibody

Immunocytochemistry/ Immunofluorescence: Aldolase A Antibody [NBP1-87488]

Immunocytochemistry/ Immunofluorescence: Aldolase A Antibody [NBP1-87488]

Immunocytochemistry/Immunofluorescence: Aldolase A Antibody [NBP1-87488] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Aldolase A Antibody Aldolase A Antibody

Aldolase A Antibody [NBP1-87488] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Aldolase A Antibody [NBP1-87488] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Aldolase A Antibody Aldolase A Antibody

Aldolase A Antibody [NBP1-87488] -Staining of human kidney shows moderate cytoplasmic positivity in cells in distal tubules.

Aldolase A Antibody [NBP1-87488] -Staining of human kidney shows moderate cytoplasmic positivity in cells in distal tubules.

Applications for Aldolase A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aldolase A

Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

Alternate Names

ALDA, aldolase A, fructose-bisphosphate, EC 4.1.2.13, fructose-1,6-bisphosphate triosephosphate-lyase, fructose-bisphosphate aldolase A, GSD12, Lung cancer antigen NY-LU-1, MGC10942, MGC17716, MGC17767, Muscle-type aldolase

Gene Symbol

ALDOA

Additional Aldolase A Products

Product Documents for Aldolase A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aldolase A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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