Aldolase B Antibody (OTI3C6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00750

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3C6

Concentration

0.77 mg/ml

View all Aldolase B Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ALDOB(NP_000026) produced in HEK293 cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

39.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aldolase B Antibody (OTI3C6)

Western Blot: Aldolase B Antibody (OTI3C6) [NBP2-00750]

Western Blot: Aldolase B Antibody (OTI3C6) [NBP2-00750]

Western Blot: Aldolase B Antibody (3C6) [NBP2-00750] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aldolase B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aldolase B.
Immunohistochemistry-Paraffin: Aldolase B Antibody (3C6) [NBP2-00750] Staining of paraffin-embedded Human liver tissue using anti-Aldolase B mouse monoclonal antibody.
Flow Cytometry: Aldolase B Antibody (OTI3C6) [NBP2-00750]

Flow Cytometry: Aldolase B Antibody (OTI3C6) [NBP2-00750]

Flow Cytometry: Aldolase B Antibody (3C6) [NBP2-00750] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Aldolase B antibody, and then analyzed by flow cytometry.
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Applications for Aldolase B Antibody (OTI3C6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.77 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aldolase B

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq].

Alternate Names

ALDO2, aldolase B, fructose-bisphosphate, EC 4.1.2.13, fructose-bisphosphatase, fructose-bisphosphate aldolase B

Entrez Gene IDs

229 (Human)

Gene Symbol

ALDOB

Additional Aldolase B Products

Product Documents for Aldolase B Antibody (OTI3C6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aldolase B Antibody (OTI3C6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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