ALG8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47550

Novus Biologicals, part of Bio-Techne
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NBP2-47550
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ALG8 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: AVRECCKCIDGKKVGKELTEKPKF

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ALG8 Antibody

Western Blot: ALG8 Antibody [NBP2-47550]

Western Blot: ALG8 Antibody [NBP2-47550]

Western Blot: ALG8 Antibody [NBP2-47550] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.
Immunocytochemistry/ Immunofluorescence: ALG8 Antibody [NBP2-47550]

Immunocytochemistry/ Immunofluorescence: ALG8 Antibody [NBP2-47550]

Immunocytochemistry/Immunofluorescence: ALG8 Antibody [NBP2-47550] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ALG8 Antibody [NBP2-47550]

Immunohistochemistry-Paraffin: ALG8 Antibody [NBP2-47550]

Immunohistochemistry-Paraffin: ALG8 Antibody [NBP2-47550] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.
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Applications for ALG8 Antibody

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100-1:2000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALG8

ALG8 - asparagine-linked glycosylation 8 homolog (yeast, alpha-1,3-glucosyltransferase)

Alternate Names

alpha-1,3-glucosyltransferase), asparagine-linked glycosylation 8 homolog (S. cerevisiae, asparagine-linked glycosylation 8 homolog (yeast, asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S.cerevisiae), Asparagine-linked glycosylation protein 8 homolog, CDG1H, dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase, Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase, dolichyl-P-glucose:Glc1Man9GlcNAc2-PP-dolichyl-alpha-1,3-glucosyltransferase, EC 2.4.1, EC 2.4.1.-, HUSSY-02, MGC2840, probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene Symbol

ALG8

Additional ALG8 Products

Product Documents for ALG8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ALG8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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