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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Bovine

Applications

Validated:

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3A9

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Alix-GST fusion protein

Reactivity Notes

Predicted cross-reactivities: Rat, Xenopus, Mouse, Human

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

NB100-65678 recognizes apoptotic-linked-gene-product 2 (ALG-2) interacting protein X (ALIX), a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Alix Antibody (3A9)

Western Blot: Alix Antibody (3A9)BSA Free [NB100-65678]

Western Blot: Alix Antibody (3A9)BSA Free [NB100-65678]

Western Blot: Alix Antibody (3A9) [NB100-65678] - B. Western blot for the exosomal marker Alix and Nef in cells and exosomes (exNef). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31344124) licensed under a CC-BY license.
Western Blot: Alix Antibody (3A9)BSA Free [NB100-65678]

Western Blot: Alix Antibody (3A9)BSA Free [NB100-65678]

Western Blot: Alix Antibody (3A9) [NB100-65678] - Western blot analysis of HeLa whole cell lysate probed with MOUSE ANTI ALIX F(ab')2 Rabbit anti Mouse IgG:HRP (STAR13B)
Alix Antibody (3A9)

Western Blot: Alix Antibody (3A9) [NB100-65678] -

Western Blot: Alix Antibody (3A9) [NB100-65678] - Nef-containing exosomes deliver Nef to macrophages.A—Size distribution of the extracellular vesicles secreted by HEK293 cells determined by EM; Inset–EM micrograph of the vesicles; bar– 200 nm. B—Western blot for the exosomal marker Alix & Nef in cells & exosomes (exNef); C—Western blot for the indicated amounts of rNef & in a typical preparation of exNef (10 μg of exosomal protein); D, E–Time-course of exosome uptake quantitated by confocal microscopy; F–Time-course of exosome uptake quantitated by fluorimetry; percentage of added exosomes that was taken up is shown; G–Cells were incubated with exosomes for 48 h, excess exosomes was washed out & cells incubated for the indicated periods of time in exosome-free medum; retained fluorscence of the exosome stain PKH67 was assessed using confocal microscopy; H–Visualisation of Nef-GFP inside the cells after exposure to exNef-GFP (5 μg/ml of exosomal protein) after staining with anti-GFP antibody. Scale bars– 10 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31344124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Alix Antibody (3A9)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Use in Flow Cytometry reported in scientific literature (PMID:34298673). For IHC - This product does not require protein digestion pre-treatment of paraffin sections.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alix

ALG2 interacting protein X is a Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. It may play a role in the regulation of both apoptosis and cell proliferation.

Alternate Names

AIP1DRIP4, ALG-2 interacting protein 1, ALG-2 interacting protein X, ALG-2-interacting protein 1, alinx, ALIX, apoptosis-linked gene 2-interacting protein X, dopamine receptor interacting protein 4, Hp95, KIAA1375, MGC17003, PDCD6-interacting protein, programmed cell death 6 interacting protein, programmed cell death 6-interacting protein

Entrez Gene IDs

10015 (Human); 18571 (Mouse); 501083 (Rat)

Gene Symbol

PDCD6IP

UniProt

Additional Alix Products

Product Documents for Alix Antibody (3A9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Alix Antibody (3A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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